10 Questions 25 Answers 0 Followers
Questions related from Prem Rajak
I want to quantify OP pesticide contents in animal tissues using HPLC-UV technique. Please suggest suitable protocol(s) of the sample preparation for the above purpose with references. with...
17 September 2017 6,642 7 View
I am trying to standardize Lipid per-oxidase assay using TBARS method. At the end of the assay, when I am recording OD of the final pink coloured product, the reading is fluctuating and is...
30 June 2016 8,414 6 View
I want to do Lipid peroxidation assay according Ohkawa et al 1979. I want to prepare standard curve using tetra-methoxy-propane (TMP). Whether, I have to prepare the standard curve directly by...
16 July 2015 9,705 3 View
I performed catalase assay according to A.K Sinha with modification. I took 1 ml of reaction mixture containing 20uL sample, 780uL dH2O, 0.1M Na-P buffer (final conc. 0.01M) and 100uL H2O2 soln...
05 May 2015 5,719 3 View
I want to do micronucleus test to detect chromosomal fragments in the mid gut of Drosophila Larvae. Kindly share valuable protocol related to this. Thanking you in advance.
12 February 2015 1,404 1 View
I want to do RT PCR of mRNAs of Heat Shock Proteins related to Drosophila tissue system. What is the procedure of sample preparation for RT PCR in insect tissue system?
24 January 2015 9,093 1 View
Is there any formula for the catalase assay to convert the O.D into umol/min/mg?
30 December 2014 7,898 13 View
In a tissue sample, I got only 70% viable cells. Is it acceptable to carry out Comet assay in this tissue sample?
29 December 2014 9,540 3 View
I recently performed a comet assay and therefore I need a software for scoring purpose. Kindly help me in this matter. Thanking you.
21 December 2014 9,536 20 View
Recently, I did a comet assay to observe DNA damage. But I faced some difficulties during the whole procedure. Kindly suggest some convenient ways to flatten the agarose layer on the slide. Can I...
10 December 2014 4,511 8 View
