Prem Rajak

6 Questions 18 Answers 0 Followers

Questions related from Prem Rajak

Can any one suggest protocol of sample preparation for quantification of organophosphate pesticide in animal tissue using HPLC-UV technique?

I want to quantify OP pesticide contents in animal tissues using HPLC-UV technique. Please suggest suitable protocol(s) of the sample preparation for the above purpose with references. with...

17 September 2017 6,596 7 View

What is the method for standard curve preparation for Lipid peroxidation assay?

I want to do Lipid peroxidation assay according Ohkawa et al 1979. I want to prepare standard curve using tetra-methoxy-propane (TMP). Whether, I have to prepare the standard curve directly by...

16 July 2015 9,656 3 View

How can I determine catalase activity in insect homogenate?

I performed catalase assay according to A.K Sinha with modification. I took 1 ml of reaction mixture containing 20uL sample, 780uL dH2O, 0.1M Na-P buffer (final conc. 0.01M) and 100uL H2O2 soln...

05 May 2015 5,657 3 View

How can I make samples of Drosophila tissue (for HSP mRNAs) for RT PCR analysis?

I want to do RT PCR of mRNAs of Heat Shock Proteins related to Drosophila tissue system. What is the procedure of sample preparation for RT PCR in insect tissue system?

24 January 2015 9,034 1 View

How can I do a catalase assay without preparing any standard curve?

Is there any formula for the catalase assay to convert the O.D into umol/min/mg?

30 December 2014 7,825 13 View

Can anyone provide a general software for a comet assay?

I recently performed a comet assay and therefore I need a software for scoring purpose. Kindly help me in this matter. Thanking you.

21 December 2014 9,491 20 View