How Triton-x removes SDS from SDS-Polyacrylamide gel?

I came to know about removal of SDS from Polyacrylamide gel is done by Triton-X, but what is the exact mechanism of its removal. How Triton-x does that? Thank you

03 February 2021 7,206 2 View

How can we differentiate crystalline peak to an amorphous peak of XRD ?

When characterized with XRD , my prepared sample show peaks which are broader(bottom) than crystalline peaks but unlike amorphous materials, have sharp top. Is there any limit of intensity or full...

15 December 2020 671 4 View

Can enzyme that is extracted from Aspergillus niger be used in the range of 4-40% v/v for optimization of veggie juices like broccoli??

09 December 2020 7,083 3 View

I need help in peptide oxidation during deprotection group removal?

Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...

03 December 2020 3,225 2 View

Regarding FAR, FRR, EER curves?

I have the image database, but I need to calculate the FAR, FRR, and EER and plot them. I am unable to understand how to plot them as they are the images... I am unable to make a program using the...

05 October 2020 3,459 3 View

Can anyone help me out to find SMA connector for a 5g millimeter-wave antenna of dimension 10*10 mm ?

Hello everyone. I am looking for an SMA connector for a 5g millimeter-wave antenna of dimension 10*10mm. The frequency range of operation is up to 40 GHz. If anybody from India is working in this...

29 August 2020 5,275 5 View

How to convert sdf file to pdb or mol2 file ?

I am using Autodock4 for protein-ligand interaction. I need a pdb or mol2 file for ligand. I have downloaded sdf file and convert it in pdb and mol2 file by using save as option. But these files...

23 February 2020 8,819 7 View

Wildtype plant DNA concentration and dilution?

I have 10 rice samples which I'm testing with housekeeping primers. 9 of them are supposed to be transgenic. And 1 is wildtype. I did a PCR with all of them, in which I had a band with all the...

09 February 2020 9,323 3 View

How to quantify biofilm biomass, maximum thickness, and surface to volume ratio from CLSI image, using Image J??

I tried using COMSTAT plugin in Image J, but it is unable to analyse .czi format. Can anyone help?? Or is there any other way to analyse biomass, maximum thickness, and surface to volume ratio...

03 December 2019 3,634 4 View

How do devices live in their own reality based on the information that has been derived?

This question is for my thesis which exploring the internet infrastructure, internet of things and aspects of object-oriented ontology in building device-device relationships.

22 September 2019 2,367 2 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Why CHi^2 is reducing if I divide the intensity by a constant factor?

I was refining my XRD data in FullProf. Highest intensity was 400000. The fitting was good but still the Chi^2 value is very high (0.174E+04) I divided the intensity by some constant factor (say...

17 February 2021 3,383 5 View

How do I refine a specific region of my protein model so that it perfectly superposes with a template peptide PDB with the same sequence?

I have a protein model and want to replace a 12-amino acids-long region within the center of the model with those of a PDB file containing a 12-amino acids long peptide of the same sequence. This...

02 February 2021 779 2 View

Is it possible to do Reitveld Refinement on single crystal XRD data?

I tried to do single-crystal Rietveld Refinement on my single-crystal XRD data using the option "refinement on single-crystal data/ integrated intensity data" in Fullprof but it is giving error...

30 January 2021 7,813 3 View

How much Ti and B (in ppm) is required for effective grain refinement and why?

To have the desired grain size in 6061 and 6063 Al alloy, what should be the minimum % of Ti and B pick up and what is the chemistry/metallurgy behind that?

07 January 2021 9,305 2 View

How to write a PHD proposal for a predefined research?

Hello everyone, I am aiming to write a PHD proposal of 1000 words. The research is described by the supervisor. That means, research questions, aim, and instruments are well defined by the...

01 January 2021 6,291 7 View

Rietveld refinement. Refined cell parameters are wrong?

I am trying to refine my XRD data with Fullprof software. Although I got low values for R Bragg and Chi 2 factors, when I insert new cell parameters as phase, they don't fit all peaks. In fact,...

18 December 2020 9,322 7 View

Modelling of proton disorder from SCXRD data?

Hi Is it possible to model a proton disorder from the single crystal X-ray diffraction data? We don't have access to neutron diffraction. #crystallography #SCXRD #refinement of structures Thanks

12 December 2020 9,507 3 View

How to perform Rietveld refinement of doped bulk powder sample using GSAS-2?

I have XRD data of a doped powder sample. How can I perform the Rietveld refinement using GSAS-2. When I use cif file of doped powder, symmetry is lost in the refining process. How to perform...

10 December 2020 9,717 4 View

Is the rarity of earth metals and the refinement of the metals with harsh chemicals stalling the acceptance of electric vehicles (EV)?

Some of the metals used to build the battery pack for a hybrid car or electric vehicle can only be mined in certain areas of the world. Is this slowing down the acceptance of the vehicles? Thank...

08 November 2020 934 3 View