How Triton-x removes SDS from SDS-Polyacrylamide gel?

I came to know about removal of SDS from Polyacrylamide gel is done by Triton-X, but what is the exact mechanism of its removal. How Triton-x does that? Thank you

03 February 2021 7,206 2 View

How can we differentiate crystalline peak to an amorphous peak of XRD ?

When characterized with XRD , my prepared sample show peaks which are broader(bottom) than crystalline peaks but unlike amorphous materials, have sharp top. Is there any limit of intensity or full...

15 December 2020 671 4 View

Can enzyme that is extracted from Aspergillus niger be used in the range of 4-40% v/v for optimization of veggie juices like broccoli??

09 December 2020 7,083 3 View

I need help in peptide oxidation during deprotection group removal?

Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...

03 December 2020 3,225 2 View

Regarding FAR, FRR, EER curves?

I have the image database, but I need to calculate the FAR, FRR, and EER and plot them. I am unable to understand how to plot them as they are the images... I am unable to make a program using the...

05 October 2020 3,459 3 View

Can anyone help me out to find SMA connector for a 5g millimeter-wave antenna of dimension 10*10 mm ?

Hello everyone. I am looking for an SMA connector for a 5g millimeter-wave antenna of dimension 10*10mm. The frequency range of operation is up to 40 GHz. If anybody from India is working in this...

29 August 2020 5,275 5 View

How to convert sdf file to pdb or mol2 file ?

I am using Autodock4 for protein-ligand interaction. I need a pdb or mol2 file for ligand. I have downloaded sdf file and convert it in pdb and mol2 file by using save as option. But these files...

23 February 2020 8,819 7 View

Wildtype plant DNA concentration and dilution?

I have 10 rice samples which I'm testing with housekeeping primers. 9 of them are supposed to be transgenic. And 1 is wildtype. I did a PCR with all of them, in which I had a band with all the...

09 February 2020 9,323 3 View

How to quantify biofilm biomass, maximum thickness, and surface to volume ratio from CLSI image, using Image J??

I tried using COMSTAT plugin in Image J, but it is unable to analyse .czi format. Can anyone help?? Or is there any other way to analyse biomass, maximum thickness, and surface to volume ratio...

03 December 2019 3,634 4 View

How do devices live in their own reality based on the information that has been derived?

This question is for my thesis which exploring the internet infrastructure, internet of things and aspects of object-oriented ontology in building device-device relationships.

22 September 2019 2,367 2 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

Can any scholar assist me with an r code for calculating average bias for each parameter of a continuous distribution via simulation?

I want to perform a simulation study to examine the consistency property of parameters of a new distribution through the quantile function. The simulation study will contain many replicates (say...

10 February 2021 180 3 View

Do the introduced salts (NaOH/ HCl) influence the spectroscopic shifts of the fluorescence excitation-emission matrix?

During the resin (XAD-8) fractionation of wastewater salts (NaOH or HaCl) are used for acid or base elutions/pH adjustments. Since the amount of introduced salt is minimal, so is it required to...

08 February 2021 9,489 2 View

Is there any minimum number for study to perform publication bias tests?

Whether funnel plot or egger test, is there any minimum number for study to perform publication bias tests?

26 January 2021 5,741 3 View

Do anyone have come across write up on "Technology on De-biasing" ?

De-biasing is a term applied in psychology, behavior, and management decision process. Now it has some connection with technology. Technology is used for leveraging on the reduction of impacts of...

21 January 2021 4,572 2 View

Why is anionic exchange chromatography more common than cationic exchange chromatography?

Is it true that there are more negative charged proteins?

18 January 2021 8,302 2 View

Multiple measures for single outcome - how to manage for meta-analysis?

Hi all, Currently doing a systematic review and meta-analysis. I am currently calculating standard mean differences (SMDs) for depression in people with X disease vs. controls without...

01 January 2021 4,707 5 View

How to synthesize pyrrolium ionic salt?

I am keenly interested in the synthesis of heteroaromatic ionic salts for use as an organic template. I want to synthesize alkyl pyrrolium chloride or bromide through a simple addition reaction...

30 December 2020 2,778 3 View

What should I do if I found publication bias?

I conducted a meta-analysis of proportion on R. After I obtained the funnel plot little asymmetry, I used Egger's regression test and rank correlation test to evaluate the publication bias: for...

25 December 2020 5,116 7 View

How to estimate prediction bound (interval) ?

Prediction bounds are relatively important when an interval considering the error of the regression model and not only sampling error comparing to the confidence interval.

13 December 2020 8,617 4 View