Hello, 

I am interested in counting the live and dead differentiated SH-SY5Y cells by using live/dead assay for my neurotoxic experiment. However, the dead SH-SY5Y cells are easier to float or sucked up during media change. I have been using pdl plate and cellbind plate, and it seems the cells do not like the plate, and cluster into ball shape.

I am wondering does anyone has experience in seeding SH-SY5Y cells and what kind of plate should I seed SH-SY5Y cells so I can get an accurate count for live/dead ratio?

Thanks

THomas

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