How to pick up top 3 genes from the top genes in two different comparisons in DESeq analysis?

19 January 2024 0 2K Report

I have got my DESeq results back - heatmaps of top 30 genes for each comparison.

- I have got top 30 genes from comparison group I, these 30 are highly expressed in condition A but not in condition B.

- I have also got top 30 genes from comparison group II, these 30 are highly expressed in condition A but not in condition C.

- I have then found 14 overlapped genes - these 14 are highly expressed in condition A but not in condition B or C.

The question is, how do I pick up top 3 from these 14? as I just need top 3 for my further experiment.

Can I just pick by log2foldchange or by the mean of the gene rank in each comparison group or by any other factor? Thank you very much.

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Science method

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