The root-knot nematodes, Meloidogyne spp. are easily obtainable. Look for galls on the roots of plants. Most of the important crops are parasitized by these nematodes.

Other plant-paratisitic nematodes are easily found as well. The Baermann funnel can be used to isolate them from the soil.

Nematode diagnosis requires efficient recovery of the plantparasitic

nematodes from the plant or soil sample. The easiest

and most simple method is to submerge a plant sample

in water in a Petri dish and directly select the nematodes

for further identification using the microscope.

The easiest nematode to obtain is the root knot nematodes, Meloidogyne spp. which are dominant here in North Africa and as Jonathan Eisenback mentioned you can recognize them from the galls on the roots. You must take care while washing soil sample because you might lose your nematodes during this process. You can know the patches where the parasitic nematodes found from their symptoms. Symptoms caused by nematode infestation differ depending on the crop and the type of nematode pest. Nematode damaged plants usually occur in patches or along a row. Infested plants may appear stunted, wilted and unthrifty. Nematode feeding also causes symptoms such as yellowing, stem twisting, crown and bulb bloating.

Later if you wish to know how to keep them in Lab, I could help you.

Thank you for the responses. Mona, how?

Helicotynchus, Hoplolaimus, Tylenchus etc. are very common.

Actually it depends on the plant species that you are studying. Some plant-parasitic nematodes are specific for one host. Some of the most common Nematodes were mentioned above

Root-knot nematodes are the most destructive plant parasitic nematode in the Philippines. The father of plant nematology in the Philippines is Romulo Davide.

He has retired, but may be able to help you.

Root knot nematode, Meloidogyne Spp are one of the most destructive pest cause huge loss to the agriculture crops.Most importantly they can be identified easily on the basis of galls infected the root of the plants.

Phytoparasitic Nematodes presence in rhizosphere basically depend on the host plant. The root knot Nematodes Meloidogyne spp.have a wide host range and are the most destructive plant pathogens. If it is a vegetable crop the commonly found genera are Meloidogyne spp., Ditylenchus spp., Tylenchus spp., Xiphinema spp., Helicotylenchus spp., Pratylenchus spp., Tylenchorhynchus spp., Criconemoides spp. Are the common Nematodes that are easily found in almost every crop vicinity few are ectoparasites or migratory endoparasites while the Meloidogyne (Root Knot Nematode) is sedentary endoparasites whose juveniles and males can only be found in soil but females reside in root within knots along with eggs.

Probably Root-knot nematodes (Meloidogyne spp.) are the best option since they are globally distributed and also it is easy to maintain and/or propagate them in the greenhouse.