I am performing neutrophil phagocytosis assays (with pHrodo bioparticles), and need to incubate the neutrophils for 2 hours in culture media before the assay. I am using RPMI with L-glutamine, and I get no phagocytosis. I know that adding fetal bovine serum would probably help, but my research is looking at different leptin levels, so I am reluctant to use serum. Is there anything specific I should be adding? Or is FBS the only way to get this to work?
Caitlin,
We also noticed that there is leptin in the serum... but had to do it this way. I will ask my post-doc if he has looked trying to do this in any other way.
On another note, we are seeing some interesting data with Q223R and neutrophils. Send me an email and maybe we can set up a time to talk and discuss the data.
Rajat
Dear Caitlin,
Try adding 10microM of Carbachol/ACh/Pilocarpine....anyone of these 3 and see. Post me a frozen Chocolate, if it works. :)
Best,
Mannan
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