Our research group normally collects brain samples from mice after drug treatment to determine drug levels, ie. to confirm BBB penetrability. Collections involve sacrificing the mouse by CO2 asphyxiation and cervical dislocation, and subsequent removal of the brain for snapfreezing.
My question is, does the amount of blood in the brain after collection contribute to levels detected in the brain after LC-MS/MS? Should I be doing cardiac perfusions with PBS to flush out the blood before perfusion?
Samir Ranjan Panda
Hi Caitlin Ung
Yes, it is always a good practice to do PBS perfusion (Flow Rate: 1ml/min), which helps to remove blood from the brain tissue, that can interfere with the measurement of drug concentrations by LC-MS/MS.
Thanks,
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