What N terminal signal sequence can be added for expression of a membrane protein in insect cell using baculovirus expression system?

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How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

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Annemarie Honegger

This article will probably answer your question

Article Vectors for Expression of Signal Peptide-Dependent Proteins ...

Dominique Liger

Hi there,

If your membrane protein of interest is eukaryotic, it should be properly processed without the need of adding any further signal peptide encoding sequence.