What N terminal signal sequence can be added for expression of a membrane protein in insect cell using baculovirus expression system?

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Interaction between GPCR and Gproteins

Can anyone suggest me how to predict interaction between a gpcr and a GPROTEIN bioinformatically?

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What are the countries most affected by extreme rainfall as a result of climate change?

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Why might my GFP tag be dissociating from my plasmid after transfection?

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How to accurately quantify chitin?

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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What is the most suitable server for predicting protein structures in bacteria?

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Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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POlymer+DMSO solution and moisture??

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How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

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Annemarie Honegger

This article will probably answer your question

Article Vectors for Expression of Signal Peptide-Dependent Proteins ...

Dominique Liger

Hi there,

If your membrane protein of interest is eukaryotic, it should be properly processed without the need of adding any further signal peptide encoding sequence.

Shilpa Sarkar

Dominique Liger it is an entamoeba gpcr