Shilpa Sarkar

4 Questions 4 Answers 0 Followers

Questions related from Shilpa Sarkar

How to extract small molecules from cell culture media for LC-MS ?

I want to find a chemoattractant present in spent media which possible is a small unknown molecule. Please suggest me ways to extract the desired fraction and remove the other interfering...

06 June 2019 3,673 3 View

What is cleavage position of the honey bee melitin signal sequence in sf9 cells?

I am trying to express a membrane receptor of entamoeba origin in Sf9 cells. I will be using the HBM signal peptide for targeting my protein in the insect cells. the HBM is generally cleaved after...

09 September 2018 720 1 View

What N terminal signal sequence can be added for expression of a membrane protein in insect cell using baculovirus expression system?

i am trying to express a membrane receptor in the insect cell system. what signal sequence should i use for expression of the receptor in the insect cell membrane?

09 September 2018 3,513 3 View

Interaction between GPCR and Gproteins

Can anyone suggest me how to predict interaction between a gpcr and a GPROTEIN bioinformatically?

01 January 1970 3,981 4 View