Creating incubation chambers on a glass coverslip-microarray?

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About maintenance of Barnstead water purification system?

Hi everyone, I have a question regarding the maintenance of water purification system. So I replaced the exhausted cartridge with the new ones, and those cartridges exhausted after 5 months...

08 September 2017 5,302 2 View

Any suggestions about PEGylated peptide purification?

Hello everybody My peptide has MW of 5 kDa, to be conjugated with PEG-SH 10 kDa via SH-maleimide interaction at high pH. This is not a specific binding, so, after fisnishing reaction, I have 3...

03 April 2015 7,756 6 View

Does anyone experience citrate gold nanoparticle nonspecific binding?

I'm trying to couple carboxylated nanoparticle with a biomolecule which has thiol group at the end, later I use that thiol group to couple with citrate gold nanoparticle to make a fluorescent...

31 December 2014 4,065 8 View

What methods of quantification of MMP-9 secretion in cells exist?

Hi everyone, My name is Nguyen Diem and I'm new to cell research. Now I'm working on a biosensor that could measure the secretion of metalloproteinase-9 in cancer cell. I'd like to ask if we can...

10 November 2014 3,211 4 View

Should we purify Metalloproteinase 9 (MMP-9) after being activated by p-aminophenylmercuric acetate?

Hello, is there anybody currently working on cell-secreted protease which is mainly focused on Matrix Metalloproteinase 9? I'm new to cell manipulation research. I'd like to ask whether we should...

09 October 2014 9,375 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View