Diem Phuong Nguyen

6 Questions 10 Answers 0 Followers

Questions related from Diem Phuong Nguyen

About maintenance of Barnstead water purification system?

Hi everyone, I have a question regarding the maintenance of water purification system. So I replaced the exhausted cartridge with the new ones, and those cartridges exhausted after 5 months...

09 September 2017 5,332 2 View

Creating incubation chambers on a glass coverslip-microarray?

Hello everyone, So I have a microarray on a glass coverslip (1.5 mm thick). I would like to create incubation chambers on it so I could use one array for several incubation targets. I did some...

01 January 2017 9,686 2 View

Any suggestions about PEGylated peptide purification?

Hello everybody My peptide has MW of 5 kDa, to be conjugated with PEG-SH 10 kDa via SH-maleimide interaction at high pH. This is not a specific binding, so, after fisnishing reaction, I have 3...

04 April 2015 7,785 6 View

Does anyone experience citrate gold nanoparticle nonspecific binding?

I'm trying to couple carboxylated nanoparticle with a biomolecule which has thiol group at the end, later I use that thiol group to couple with citrate gold nanoparticle to make a fluorescent...

01 January 2015 4,105 8 View

What methods of quantification of MMP-9 secretion in cells exist?

Hi everyone, My name is Nguyen Diem and I'm new to cell research. Now I'm working on a biosensor that could measure the secretion of metalloproteinase-9 in cancer cell. I'd like to ask if we can...

11 November 2014 3,262 4 View

Should we purify Metalloproteinase 9 (MMP-9) after being activated by p-aminophenylmercuric acetate?

Hello, is there anybody currently working on cell-secreted protease which is mainly focused on Matrix Metalloproteinase 9? I'm new to cell manipulation research. I'd like to ask whether we should...

10 October 2014 9,457 3 View