Any suggestion to molecular approaches or methods (apart from RT-PCR) that can be used for analysis and identification of probiotic strains that were fed to broiler chicken. There was growth difference which could be attributed to the probiotics but a molecular proof of the GIT is needed.
Go for metagenomic analysis of gut content then you will come to know the types of microorganisms present in it
You can use standard typing methods like RAPD and PFGE for the identification of your strain.
See papers:
The effect of wheat prebiotics on the gut bacterial population and iron status of iron deficient broiler chickens.Tako E, Glahn RP, Knez M, Stangoulis JC.Nutr J. 2014 Jun 13;13:58. doi: 10.1186/1475-2891-13-58.
Intra-amniotic administration and dietary inulin affect the iron status and intestinal functionality of iron-deficient broiler chickens. Tako E, Glahn RP. Poult Sci. 2012 Jun;91(6):1361-70. doi: 10.3382/ps.2011-01864.
Thank you all. Really helpful referring me to metagenomic analysis.
- Badges
- Science method