I have been utilizing Polytetrafluoroethylene (PFTE) membranes with 0.45 μm pore size (Millicell cell culture inserts) for impression cytology. I am using PBS with 0.5% PFA as a medium. However, I am encountering difficulties in detaching the cells from the membrane.
Despite attempting agitation with a pipette tip, I am unsuccessful in fully dislodging all the cells from the membrane.
I am curious whether the material and pore size of the membranes could be contributing to this issue. For instance, I noticed that Eyeprim employs polyethersulfone 0.2 μm membranes.
Would anyone be able to provide any insights or recommendations regarding membrane selection for optimal cell retrieval?
Does 0.5% PFA have any effect on the membrane? Does it cause stickiness? Should I consider using a lower concentration of PFA?
Thank you
Soumen
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