What marker can I use to sort macrophages from a mixed white blood cell population using flow cytometry??

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Jelili Mustapha

Thanks John, that's helpful.

Ann-Kathrin Gaiser

If you would like to differentiate macrophages from monocytes, I would suggest to use CD68.

but be careful. Despite it is a "surface marker", you need to permeabilize the cells for staining because of large intracellular pools.

In order to differentiate between M1 and M2 macs, you might have a look at our publication:

Article Carboxyl- and Amino-Functionalized Polystyrene Nanoparticles...

Good luck!