I do a microbe culture from waste metalworking fluid using blood agar medium at 37C, and I found a colony as like as the picture in the attachment.
Any one can help me to figure out what is it?
nb: the reddish dot will appear after 24-48hr incubation, and the white colony appear after 3 days.
Thanks!
Hello, looks like fungi or actinomyces, further study needs to be performed for the identification, first start from microscopy, than you can continue by biochemical tests or by PCR.
It may be Actinomycetes. It needs microscopy test. So the picture is not clear enough to identify it.
Might be fungi... probably actinomycetes...but maybe a more detailed microscopy will help..
the colony characteristics resembles fungi belonging to the group filamentous fungi or Ascomycete group of fungi.
Further we have to check for microscopic studies by lactophenol cotton blue staining or simply seeing under the microscope reveals their characteristics and spores and at times colorful spores can be visualised
Thanks to Karen A. Darbinyan , Yehya A. Salih , David Adeiza Zakari , and Aditee Chintankumar Pandya . I will prepare for microscopy observation.
Phil Geis The great scientist and engineer is the one who able to determine and give a correct judgement with limited data and observation. Not only because of the great result and beautiful data! where especially in industrial field with very limited characterization and identification equipment. Glad if you are an experienced! I do ask because I'm learning and looking for a good deeds. Thanks!
Daniel - folks here are eager to help and often offer comments that are not "great" but obvious or unjustified guesses (recall the post of named species that was kllkater deleted). . Here not only based on a blurry picture but from person who is ladmittedly learning and may well have limited knowledge of aseptic technique for sampling, inoculation, incubation etc. leaving the question of contamination/lab error. In any caswe, you have a fungus - microscopy will tell you nothing more as you are new this this are..
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