if the reaction in your assay emits luminescence, as is the case with the Cell Titer Glo assay for example, you should use 96-well plates with white walls.

black walls are best used for fluorescence/ fluorogenic assays.

Good luck.

Thank you Tina

the plates don't need to be ultra low attachment, unless you usually use low attachment plates for your cell line. I use tissue culture-treated  96-well plates with white walls and i have gotten good results.

Thanks again Tina for your information. I will try to grow the tumor cells in tissue culture-treated 96-well plates with white walls.

Do those plates need to be black with white walls or just white?