Hi,
I have been doing some experiments to reveal cytotoxic/antiproliferative effects of the new compounds in the cancer cell lines. After 24 hr incubation with compounds in Hep3B cells, I realized a specific structure on the wells with different compounds even my positive control (adriamycin), but not on control wells. So I am attaching an image of the wellhole shaped structure. Can you give an idea what this structure is or looks like? Thank you
Hi Santanu,
Thank you for your advice. Actually, I am seeing this structure very long time, I always thought it was my mistake of spreading cells. Still, it keeps pop up on my treatments. I am pretty sure, it is not a contamination. The structure I am interested is not a single cell. It is a group of cells. There are hundreds of cells become to form this structure.
Thank you
see article of Levanon in Ultrastruct Pathol and maybe ask these authors on this cell line?
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