Best serverless deployment on Raspberry Pis cluster (swarm, k3s, openfaas, faasd, etc)?

Hi folks, I am going to deploy a cluster of Raspberry Pis (preferably, 3 b+) with OpenFaaS serverless. Previously, the OpenFaaS used to work on Swarm orchestration, but it appears deprecated...

14 January 2021 1,824 1 View

Study of metastasis in-vivo is feasible?

Dear colleagues, I am working on a research project to investigate the metastatic propagation of cancer cells, so I need to see the metastasis of these cells in-vivo. Can any help me to find an...

04 September 2020 7,714 3 View

COVID-19 and Renewable Energy?

High oil prices, environmental crises, and increasing energy demand have been the driving forces for rapid growth of renewable energy worldwide. All these forces have been weakened by COVID-19....

28 March 2020 3,752 20 View

Simple explaination for Polynomial chaos expansions (PCEs)?

Hi guys, Can anyone describe PCE in simple words, please? How can we find a PC basis and what is an appropriate sparse PC basis? Thanks in advance

17 January 2020 7,471 3 View

Does anyone know how to create a multi temporal map from different single band raster layers?

I`m wanna create a multi temporal map of built up areas in different time spans to show the urban sprawl trend in one layer. There are NDBI layers with a specific range which covers all features...

04 October 2019 9,272 3 View

Signal decomposition by wavelet synchrosqueezed transform?

Hi, I was reading an article, it used synchrosqueezed wavelet transform to decompose the following signal in its fundamental modes. Please, can anyone explain why I can't reach the same conclusion...

28 September 2019 3,650 8 View

Hyperparameter tuning of neural network?

I need a code to find the optimum configuration of a neural network. I mean the number of hidden layers and their corresponding neurons. I found the following topic but there's nothing on the...

20 July 2019 3,047 8 View

Is there any online service that perform calculation using Wien2k?

Wien2k server for calculation

06 March 2019 7,153 2 View

Is there any procedure for SIL & HAZOP (hazardous Operability) Study in gas industry?

hi i need a procedure for SIL & HAZOP study in gas refinery such as definition of nodes, deviation, safe guard and recommendation. thanks

31 January 2019 8,073 3 View

Screenshot picture quality - how to improve?

In my research paper, to bring some evidence, I need to take screenshots of my codes. To this end, I am using Screenshot tools, e.g. Free Snipping and FastStone Capture. Also, I am increasing the...

19 January 2019 7,851 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View