Is there a material that can absorb (or reflect) a light in one-way?

Hi, I have a question about reflection, transmission, and absorption of light. Is there a material that can absorb (or reflect) a light in one-way? For example, there is a substrate that has a...

01 December 2019 370 5 View

How can FeNWs be dispersed in organic solvent?

Hi, Does somebody have an idea to disperse FeNWs (or AgNWs) in organic solvents such as acetone, chloroform, and ethyl acetate? If a surfactant does the trick, please recommend me a proper...

25 September 2019 644 3 View

Has anybody faced difficulties with islets isolation from STZ induced diabetic mouse?

Recently, I am trying to isolate islets from STZ induced diabetic mice. I have tried to tweak the type of the collagenase, collagenase concentration, incubation time, stoping method..etc.. but I...

25 August 2019 2,672 1 View

Experiment conditions for ELISA using the purified protein and synthetic peptide?

Hi. I'm planning to perform ELISA to find amino acid residues which directly bind to the protein X (protein of interest). You may see the design of the experiment in the picture that I posted....

07 August 2019 2,536 3 View

How can I estimate the purity of a recombinant protein after purification process using size-exclusion chromatography (no standard product exists)?

I produced a recombinant chimeric protein from CHO cells and purified it using size-exclusion chromatography(SEC). Thankfully, I could get a clear peak for my target protein and separate it from...

23 April 2019 4,383 12 View

How high is NAFION membrane permeable to water??

Hi. I am interested in a membrane that has high permeability to water and low(appropriate) permeability to cation. And I found that NAFION can absorb and permeate water. Does anybody know the...

28 March 2019 9,014 1 View

Is there any membrane that only permeates water (not ions)?

Hi. Does anybody know a membrane that only permeates water (extremely low permeability to ions)? I would like to make a system that, when pressure is applied to a chamber in which a certain...

21 March 2019 1,663 3 View

How to determine degree of substitution of quaternized chitosan using H-NMR?

Hi, I dissolved quaternized chitosan (with GTMAC) in D2O and 1%DCl and did H-NMR to calculate the degree of substitution. However, I see a large peak at 4.7ppm (D2O?) and some overlapped peaks...

16 January 2019 4,798 3 View

Does the distance between two patch clamp electrodes influences signal-to-noise ratio?

Does the distance between two patch clamp electrodes influences signal-to-noise ratio? or any other effect on electric signal measurement? Buffer medium will be deionized water or PBS.

16 October 2017 5,320 2 View

How to calculate PM (mutual) torque and reluctance torque in PM machine by using Ansys Maxwell?

Currently, I am using two ways. Could you guys verify whether these approaches are valid to calculate PM torque and reluctance torque in PM machine? 1. Generate three-phase inductance using the...

18 December 2016 2,386 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View