I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the last amino acid (Fmoc removal). Peptide is purified and in solution phase.
The Fmoc group is, in general, rapidly removed by primary (i.e., cyclohexylamine, ethanolamine) and some secondary (i.e., piperidine, piperazine) amines, and slowly removed by tertiary (i.e., triethylamine [Et3N], N, N-diisopropylethylamine [DIEA]) amines. Removal also occurs more rapidly in a relatively polar medium (N, N-dimethyl-formamide [DMF] or N-methylpyrrolidone [NMP]) compared to a relatively nonpolar one (dichloromethane [DCM]). During solid-phase peptide synthesis (SPPS), the Fmoc group is removed typically with piperidine, which in turn scavenges the liberated dibenzofulvene to form a fulvene-piperidine adduct.
httpss://www.researchgate.net/publication/15303828_Methods_for_Removing_the_Fmoc_Group
Check https://www.peptide.com/resources/solid-phase-peptide-synthesis/n-terminal-deprotection/
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