Hi everyone, I am a master student who currently working with a program detecting amyloid β aggregation, so I need to use Abeta 42. After treating with HFIP and get a peptide film, I want to dissolve it with 1% NH4OH. But after adding the solution and pipette up and down, there is much foam producing and can't disappear in several minutes. Have you met with this situation? And can I centrifuge for a few seconds? I am afraid it would aggregate if using centrifugation, because I need the monomer form. Thanks!
Please look at the following below links which may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988635/pdf/12551_2018_Article_421.pdf
https://patentimages.storage.googleapis.com/27/1f/f7/fbbeacaa70cf61/US8497072.pdf
Thanks
The best way to deal with foam is, of course, not to cause it in the first place, by taking care when pipetting not to draw up air into the tip and to dispense gently so as not to blow out air, or by using some other means of dissolution.
You may be able to get rid of the foam once it is formed by blowing dry air over the surface. One way to do this is with a heat gun from a distance, on a relatively low heat setting, very carefully. Another way is with a squirt bottle partially filled with methanol, but with the straw through which the solvent passes to the spout removed so that only the vapor gets out.
Another thought is to put the whole thing in a vacuum chamber and draw a vacuum. This may be a bad idea; I'm not sure. It may just expand the foam.
Adam B Shapiro Thanks a lot! I will try to pipette carefully next time and try the methods you mention. (BTW, I have read a lot of your answers on researchgate, and find them useful. Special thanks for that)
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