Is it possible to transport rRNA native secondary structure through Nano channels?

if yes, does it consider microfluidic scales?

10 December 2020 5,286 2 View

Can we utilize Fresnel lens instead of dish collectors?

Hello Can we use Fresnel lens in order to concentrate the solar energy on a point (instead of using a dish collector)? If so, is there any problem or limitation about using that? Please consider...

05 March 2020 2,218 3 View

Do Matsim and GIS have a mutual interface? or any suggestion for modeling house locations in Matsim?

Dear colleagues I need the location of houses for agent-based modeling. in this regard, Open Street Map (OSM) is very useful. But OSM in our region is not very accurate and I am thinking to use...

23 February 2020 2,624 3 View

Do you know any adipogenic inhibitor apart from BADGE?

I was wondering if you could name reagents which are routinely used to inhibit adipogenesis in animals ( in my case is rat)?

14 September 2014 9,868 3 View

Can anyone help with the 2nd order boundary condition for QUICK scheme?

I have written a CFD code which uses staggered grid and QUICK discretization scheme to solve an incompressible turbulent flow problem. But I have problem with far upwind nodes at boundaries. I...

24 April 2014 4,082 2 View

How can I find the location of the aerodynamic center of a cambered airfoil?

I have a cambered airfoil and want to know how I can find its aerodynamic center experimentally or by using CFD.

11 April 2014 3,837 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View