In terms of protein expression in the HEK cell 293 F type in the form of suspension, why the cell viability after transfection decreases so much?

Mohammadhossein Shabahang @Mohammadhossein-Shabahang

14 September 2023 1 5K Report

Before transfection the viability is around 98% but exactly one day after I add the plasmid and PEI the viability of my cells decreases so much. I check the viability with the Trypan Blue 100 microliters of it with 100 microliters of cell.

Manoj Balakrishna Menon

I am not sure about your protocol, but in these kind of transfections, optimising (reducing) the DNA amounts, PEI amounts and doing transfection under antibiotic free conditions will be required to get optimum viability/transfection. Best!

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