curcumin can inhibite the activity of many cellular partners but there is no study that show a physical interaction between curcumin and these proteins and partners
Dear Boudiaf,
Attached please find a publication that discusses the transport of curcumin into plasma membrane.
Int. J. Mol. Sci. 2003, 4, 34-44
Effect of Turmeric, Turmerin and Curcumin on Ca2+
,
Na/K+
Atpases in Concanavalin A-Stimulated Human Blood
Mononuclear Cells
Hari H. P. Cohly1
, Maheshwara-Rajeswara Rao2
, Vijaya K. Kanji2
, Babu Patlolla3
, Anelle
Taylor1
, Melanie T. Wilson1
, Michael F. Angel1
, and Suman K. Das1
1
Departments of Surgery and 2
Medicine, University of Mississippi Medical Center, Jackson, MS,
39217-4505, E-mail: [email protected]
3
Department of Biology, Alcorn State University, Lorman, MS, 39096
Received: 7 June 2002 / Accepted: 30 October 2002 / Published: 31 January 2003
Abstract: Ion transport enzymes may play an important role in T cell activation. This study investigates the role of turmeric and its individual components, turmerin-and curcumin-on Ca2+ and Na/K+ adenosine triphosphatases (ATPase) in the course of T cell activation. Concanavalin A (Con A) stimulated human blood mononuclear T cell proliferation paradigm was investigated for 3, 5 and 7 day periods with different concentrations of turmeric, curcumin and turmerin. Con A-stimulated cells treated with turmeric (250, 50, 5 µg/ml) for 3 and 5 days inhibited ATPase levels when compared to base levels obtained by cells in media alone. At day 7, there was a 3-fold increase for Ca2+ATPase levels and a 2-fold increase for Na/K+ ATPase. Curcumin (250, 50, 5 µg/ml) showed the same pattern for ATPase activity s turmeric at 3 and 5 days with a 2-fold increase at day 7. Turmerin (2500, 1250, 250, 25 ng/ml) for Na/K+ ATPase activity showed an increase at day 3, a decrease on day 5, and a 2-fold increase on day 7. Ca2+ ATPase activity in the presence of turmerin showed an increase in ATPase levels at day 3 (except at 2500ng/ml where it decreased) and a decrease in day 5 (except at 25 ng/ml where it increased). Turmeric and curcumin generally inhibited Ca2+ATPase and Na/K+ ATPases in early (day 3) and intermediate (day 5) stages of mitogen stimulation. However, the effect after 7 days incubation for turmeric, curcumin and turmerin showed a marked increase up to three fold.
Keywords: Turmeric, turmerin, curcumin, Ca2+ATPase, Na/K+
ATPase.
Hoping this will be helpful,
Rafik
Dear Boudiaf,
Attached please find a publication that discusses the transport of curcumin into plasma membrane.
Int. J. Mol. Sci. 2003, 4, 34-44
Effect of Turmeric, Turmerin and Curcumin on Ca2+
,
Na/K+
Atpases in Concanavalin A-Stimulated Human Blood
Mononuclear Cells
Hari H. P. Cohly1
, Maheshwara-Rajeswara Rao2
, Vijaya K. Kanji2
, Babu Patlolla3
, Anelle
Taylor1
, Melanie T. Wilson1
, Michael F. Angel1
, and Suman K. Das1
1
Departments of Surgery and 2
Medicine, University of Mississippi Medical Center, Jackson, MS,
39217-4505, E-mail: [email protected]
3
Department of Biology, Alcorn State University, Lorman, MS, 39096
Received: 7 June 2002 / Accepted: 30 October 2002 / Published: 31 January 2003
Abstract: Ion transport enzymes may play an important role in T cell activation. This study investigates the role of turmeric and its individual components, turmerin-and curcumin-on Ca2+ and Na/K+ adenosine triphosphatases (ATPase) in the course of T cell activation. Concanavalin A (Con A) stimulated human blood mononuclear T cell proliferation paradigm was investigated for 3, 5 and 7 day periods with different concentrations of turmeric, curcumin and turmerin. Con A-stimulated cells treated with turmeric (250, 50, 5 µg/ml) for 3 and 5 days inhibited ATPase levels when compared to base levels obtained by cells in media alone. At day 7, there was a 3-fold increase for Ca2+ATPase levels and a 2-fold increase for Na/K+ ATPase. Curcumin (250, 50, 5 µg/ml) showed the same pattern for ATPase activity s turmeric at 3 and 5 days with a 2-fold increase at day 7. Turmerin (2500, 1250, 250, 25 ng/ml) for Na/K+ ATPase activity showed an increase at day 3, a decrease on day 5, and a 2-fold increase on day 7. Ca2+ ATPase activity in the presence of turmerin showed an increase in ATPase levels at day 3 (except at 2500ng/ml where it decreased) and a decrease in day 5 (except at 25 ng/ml where it increased). Turmeric and curcumin generally inhibited Ca2+ATPase and Na/K+ ATPases in early (day 3) and intermediate (day 5) stages of mitogen stimulation. However, the effect after 7 days incubation for turmeric, curcumin and turmerin showed a marked increase up to three fold.
Keywords: Turmeric, turmerin, curcumin, Ca2+ATPase, Na/K+
ATPase.
Hoping this will be helpful,
Rafik
This abstract (http://www.sciencedirect.com/science/article/pii/S0304416507002796) says that the cellular concentration of curcumin in cultured cells was directly proportional to the treatment concentration, and it was concentrated in membranes. A lack of saturability of uptake suggests a passive process of uptake, such as pinocytosis or, more likely considering the hydrophobicity and lack of water solubility of curcumin, passive diffusion across and within the lipid bilayer.
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