How we can store tonsillitis sample after tonsillectomy?

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Are the messages transmitted in classical channel between participants in quantum cryptographic protocols free from being blocked or delayed ?

In quantum cryptographic protocols, participants typically share both a quantum channel and a classical authenticated channel. Authenticated channels ensure that messages come from legitimate...

22 June 2024 7,074 3 View

How to obtain runoff volume and peak discharge value from HEC-HMS model?

Hello everyone, I am trying to create a simple model using the HEC-HMS to obtain the value of runoff volume and peak discharge for a river basin. The problem is I do not know which method...

23 March 2024 6,835 5 View

What are the requirements and steps to calculate rainfall-erosivity index, EI30 using Wischmeier and Smith (1978)?

I am trying to calculate the rainfall-erosivity index EI30 using 30-minutes interval rainfall data. For that I have a few questions on the requirement and steps for the calculation. 1. Low...

18 December 2023 2,262 0 View

How to calculate mol ratio of epoxy natural rubber latex with diacid?

Hi, I have a problem in calculating the mol ratio of ENR. This is required as I want to include additives in my compounding. But, the amount need to be in mol ratio to see equivalent amount of...

14 December 2023 3,845 2 View

Does anyone know how to perform tuckey test and compact letter display?

I have optimized the extraction of polyphenols using BBD via RSM. I carried out the extractions in triplicates and performed TPC assay on them. Hence, for each run I have 3 extraction with 3 TPC...

18 October 2023 1,211 0 View

Obtaining natural product dry powder extract using spray drying method?

I needed some guidelines for obtaining spray dried plant extract powder. I have using 50 % aqueous ethanol as my solvent and have performed liquid extraction from my plant using shaking incubator....

05 October 2023 5,478 0 View

In alpha amylase inhibitory activity, while taking UV and, do we zero the absorbance of blank or control?

I have to conduct alpha amylase inhibitory activity but I m confused while taking readings from uv-vis spectrophotometer regarding blank and control. Do we have to zero the blank and then take...

01 October 2023 4,297 1 View

What should I do if too light band get on 3% agarose gel after overnight incubation in RFLP?

The PCR product that I used shows 170 bp product in a separate gel. But also contains its recognition site according to the reference paper. I used the protocol as provided by the Thermoscientific...

25 September 2023 3,727 2 View

Factors contributing to pH fluctuation during Penicillin production by Penicillium chrysogenum?

I have observed that pH goes down during the preculture for three days, then when transfered to culture shake flasks, the pH goes up. I would like to cover all the factors that might contribute to...

27 December 2022 6,153 3 View

Determination of Total tannin Content, Condensed Tannin Content and Hydrolysable Tannin Content?

I need authentic protocols for determining total tannin content, condensed tannin content and hydrolysable tannin content. Microplate plate reader protocols will be more feasible. other protocols...

21 September 2022 8,472 2 View

Bacterial or Mycoplasma contamination or cells debris in a tonsillar explant culture?

I am facing a loss of viability in lymphocyte cells from human tonsils that I culture ex vivo. I have cultured dozens, but in the last months, small dots always appear in my media and the...

12 May 2024 2,927 3 View

Immunofluorescent staining of NK cells and B cells overlapping in tonsil but not brain?

I am testing a new antibody (CD94) to stain for NK cells in the brain. However, when I test it in tonsil tissue, it stains basically every cell in the germinal center, so I did another test with...

04 December 2023 6,361 0 View

How long should pediatric tonsilectomies recover in PACU before discharge home?

We do pediatric tonsillectomies in both inpatient and outpatient settings. Is there evidence /research on how long these patients should remain in PACU being monitored before being discharged home...

20 September 2023 8,451 0 View

Is tonsillectomy has significant effect on the immune system?

Is it possible to compensate for the effect or lack of immunity after the eradication process?

04 August 2023 9,979 2 View

Could angina and pharyngitis be reduced with coblation subtotal tonsillectomy instead of total tonsillectomy?

Given the fact that coblation subtotal tonsillectomy results in fewer complications, including reduced bleeding 24 hours after the procedure and a shorter hospital stay, in comparison to total...

15 July 2023 2,797 1 View

Malocclusion characteristics of Mouth breathing in enlarged tonsil children?

What are Malocclusion characteristics of Mouth breathing in enlarged tonsil children? Do Mouth breathing in enlarged tonsil children need treatment after tonsilectomy?

19 June 2023 5,116 1 View

What are the latest recommendations regarding the use of antibiotics after tonsillectomy ?

Tonsillectomy is one of the most common surgeries .

13 June 2023 4,978 3 View

How do you store human tonsils?

I have tried flash freezing in liquid nitrogen, then storing at -80; and slow freezing in -20, then in -80 surrounded by styrofoam, but in both cases cells weren't viable upon thaw. I was going to...

10 November 2019 2,262 1 View

How do you store human tonsils?

I have tried flash freezing in liquid nitrogen, then storing at -80; and slow freezing in -20, then in -80 surrounded by styrofoam, but in both cases cells weren't viable upon thaw. I was going to...

31 October 2019 569 1 View

What are some IHC markers that stain the liver structure?

In tonsil we normally use CD3 and CD19 to help stain the overall cross section. If I was looking at a cross section of the liver, what antibodies should I use to help me see the overall...

09 September 2018 7,521 2 View