What is the subcellular localization of cytochrome P450 epoxygenases 2C and 2J in the vascular endothelium?

More David C. Ellinsworth's questions See All

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

Hot to do MTT assay data analysis?

I have repeated an MTT assay three times using different batches of the same cell line. Each time, I tested the same treatment (different medium) and seeded the same number of cells. Additionally,...

08 July 2024 1,900 1 View

What is wrong with Site-Directed Mutagenesis?

I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...

24 June 2024 6,079 4 View

Does anyone have suggestions for this esterification?

I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...

24 June 2024 9,319 0 View

How do you add multiple nodes or cutting planes in Abaqus?

Hi, In a previous question I asked how to effectively model springs in Abaqus. However, I figured out the feature under Assembly -> springs/dashspots -> node to node Springs. I have a cylinder...

20 June 2024 892 3 View

How do you add radial spring elements in Abaqus?

Hi, I am trying to complete a buckling analysis on a tube with an external pressure. This solves itself easily by adding radial constraint on the internal edge of the tube, one axial constraint...

17 June 2024 8,165 7 View

Given that Mesozoic placentals were largely arboreal (with fingers), do you know of any studies that illustrate the finger-to-hoof transition?

I'm interested in the earliest days of placental terrestrial locomotion in large herbivorous taxa. Phenacodus seems to be transitional in this regard. Any others?

06 June 2024 5,182 2 View

How reliable is ninhydrin test in solid-phase peptide synthesis?

Hey everyone! I'm doing peptide synthesis on Rink amide resin and sometimes I need to add amino acids with secondary amines or beta amino acids. Normally I synthesize 35 to 40 mer peptides. As a...

21 May 2024 9,035 0 View

How does one generate a normalized TCSPC curve from actual photon counts versus time?

Sometimes TCSPC curves are analyzed using the actual photon counts, and sometimes they are normalized with the signal running from 0 to 1, or even from 0 to some value less than 1. What quantity...

20 May 2024 4,277 0 View

Problem when running Spin-Orbit Coupling using CASTEP. What is nlpot_prepare_precon_ks?

I am currently using CASTEP version 22.11, and I encounter the following error when running calculations with spin-orbit coupling: Error with dsytrf in nlpot_prepare_precon_ks Current trace...

19 May 2024 4,236 5 View

checklust of the vaSCULAR FLORA NATIVE TO ITALY?

a second update to the checklust of the vaSCULAR FLORA NATIVE TO ITALY

24 July 2024 2,403 0 View

What about endoform applications?

Endoform™ Antimicrobial is indicated for the management of acute and chronic wounds including partial- and full-thickness wounds, pressure ulcers, venous ulcers, diabetic ulcers, chronic vascular...

17 June 2024 820 1 View

Can someone give me some clarification re. induction of CYP3A4 and concurrent metabolism experiment?

Hi, our lab has recently started working with primary hepatocytes in 3D form. The lot we are using has high basal activity which declines as the spheroids age. We are looking to undertake drug...

21 May 2024 1,321 0 View

How can I find Retinal Images with Vascular Dementia?

Hi Everyone, Can I know any available datasets on Retinal Images with Vascular Dementia for a research project. Thank You

01 May 2024 2,750 0 View

Can someone please provide the proper methodology for the quantification of Cytochrome P450 monooxygenase in fungi?

Can someone provide a proper methodology for the quantification and identification of the cytochrome P450 monooxygenase enzyme in fungi for the enzyme assay.

25 March 2024 9,402 0 View

How can you view vascular layer of Arabidopsis roots under confocal without overstaining the outer layer? does vacuum infiltration helps?

should the temperature be raised while vacuum infiltrating?

12 February 2024 7,013 2 View

What are some well studied membrane-localized receptors, or constructs, with different or tunable dimerization affinities?

I would like to use them as controls. I am studying the dynamics of receptors on the cell surface using SMI TIRF microscopy. I am currently using CHO cells and I would like to use constructs as...

19 December 2023 3,243 0 View

How to dissolve anhydrus caffeine?

I would like to prepare a caffeine solution (1 M) for my vascular reactivity experiments (20 mM, final concentration). I have tried dissolving it in water, DMSO and ethanol; but it does not...

11 December 2023 6,784 0 View

Why are vascular catastrophes more frequent among table tennis players?

Your rigid system did not allow me to formulate my complete question: Why are vascular catastrophes more frequent among table tennis players, than chess or football players? I am not a...

09 December 2023 6,353 0 View

What is the preferred isoform of VEGF for human or mouse for inducing neovasculature?

I am grafting VEGF within a hydrogel to enhance the induction of neovasculature in the subcutaneous cavity of a mouse model. I would like to know the most suitable isoform of VEGF for this...

06 December 2023 6,753 1 View

Ilya B. Tsyrlov

Hi David,

It's ER, of course.The CYPs in the plasma membrane was 9% of that in microsomes.

Ilya

David C. Ellinsworth

Thank you Ilya, I suspected it was ER but wasn't sure. I wonder if you can recommend some references demonstrating this, particularly for vascular endothelial cells.

Many thanks for your answer

Dave