Can someone provide a proper methodology for the quantification and identification of the cytochrome P450 monooxygenase enzyme in fungi for the enzyme assay.
Can we use DCPIP (2,6-Dichloro Phenol Indophenol) reduction for quantification of crude oil degradation using fungal isolates? can someone provide with a detailed protocol for the same? Some...
01 January 2024 8,356 0 View
In one of the projects with the help of python (jupyter notebook), I applied mask RCNN for masking pomegranate fruit and classify it based on its size. During model application in real time the...
07 February 2023 7,987 3 View
We know that entropy is a measure of disorder and also we know that it always increase never decreases. Time arrow move in forward direction not in reverse. Ok now my question is when we born we...
11 November 2022 1,570 9 View
i want to built automatic start water pump for house hold near my area. where drinking water comes very early morning and without starting motor it will not come in reservoir. also the tarrain of...
10 November 2020 9,981 1 View
I have synthesized Phthalated chitosan, now want to calculate its degree of substitution, is their any method available for calculating degree of substitution by UV spectrphotometry or...
05 June 2019 8,547 2 View
How to calculate the molecular weight of monomeric unit of phthalated chitosan if the degree of deacetylation for chitosan was 80% and degree of substitution for phthalated chitosan was 12 %.
20 May 2019 4,676 2 View
i am reacting 6 hydroxyl group of chitosan with succinic anhydride, and want to know whether it gets hydrolysed during aqueous workup?
06 January 2019 1,561 0 View
Quarantine was introduced at very first time for limiting the spread of cholera and later for plague and yellow fever. But with the advent of more holistic global surveillance on all emerging and...
24 December 2013 6,526 2 View
There are slight differences in many formulations, eg., some people use 1 mL extra of MgCl to block NMDA receptors. Some use 125 mM NaCl. Obviously we have HCO3/NaHCO3 20-25 mM Glucose 2.5mM...
26 November 2012 5,018 6 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...
29 July 2024 5,015 1 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View