Problem about rat anesthesia?

I have a problem about rat anesthesia, the situation is that I have Zoletil 50 1mg/ml + xylazine hydrochloride 23.32mg/ml solution that I prepare at the same time from the same source (from the...

07 October 2020 9,140 7 View

Problems with cloudiness in cultures of suspension cells?

Good day to everyone, I am quite new to cell culture and I hope someone could help with the problem I am facing. I recently started new cultures of K562, Daudi, and NALM-16 cell lines in RPMI1640...

22 September 2019 7,711 4 View

Can I draw EEMs by Surfer (a contour mapping software)?

Hello, I am currently drawing the EEMs based on my data of lakes. During this time, I realized that PARAFAC of Matlab were commonly used in majority of EEMs studies, while I only know how to...

17 October 2018 7,773 2 View

Which is best way to convert median to mean in meta-analysis?

I'm conducting a meta-analysis, but I got a trouble in several articles that provided Median, Q1, Q3, and sample size. I have used several ways to convert Median to Mean and estimate SD, but I'm...

15 September 2018 5,824 7 View

Number of holes in the Mn 3d band in DMS (diluted magnetic semiconductor)?

Do you know about some articles calculating the band structures of DMS containing Mn in general and number of holes in Mn 3d band in particular? I need this information to calculate magnetic...

25 April 2017 7,470 3 View

How to fit M-T curve using Brillouin function in Origin?

Could you please tell me how to fit the M-T curve (below on the attached file) using Brillouin function in Origin. As far as I know, Origin doesn't have built-in Brillouin function, so I created...

06 April 2017 8,575 14 View

Smeared DNA bands in polyacrylamide gels, but not in agarose gel. Possible reasons?

20 November 2016 3,289 11 View

How can we prepare carboxylated graphene oxide without reduction?

I tried to prepare carboxylated graphene oxide by using the method in attached papers. However, from FTIR analysis, the peak of C=O group decreased and the peak of C=C increased dramatically (like...

16 April 2015 390 2 View

Does anyone know how to determine the Miller indices (hkl) of non-cubic structures such as body centered tetragonal (bct)?

The crystalline phase in my materials include bcc (Fe-Si), bct (Fe2B), and bct Fe3B. I can index the bcc (Fe-Si) in XRD or TEM, but I don't know how to index bct phase.

17 March 2014 5,054 4 View

What is the principle of creating a background curve? and How can we quantify the percentage of crystalline phase?

I use XRD to identify crystalline phases created in metallic glass film after heat treatment. In order to compare these crystalline phases, I quantify how much these phase is formed by calculating...

01 February 2014 7,941 1 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

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17 February 2021 4,217 3 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

How can I find the thermophysical properties of high temperature microencapsulated phase change materials (MPCM)?

Hi everyone. I am looking for a high temperature MPCM. More specifically, I need the thermo physical properties of a microencapsulated phase change material with the melting temperature between...

14 February 2021 3,606 2 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View