It is not clear from your question what you actually want to know. If you are looking for assaying the efficacy of these enzymes as anti-oxidant, you can perform MDA assay, commet assay, protein oxidation assay, peroxide formation assay etc.
i think what u asked is one standard formula for all the assays...... there is no standard formula for all these assays, however, each of them has different formula based on the protocol you follow and how you want to express (per gram tissue, per mg protein etc,)
how to calculate the GPx activity after getting absorbance of blank and samples? I also want to know that Decrease in absorbance is directly or inversely proportional to GPx activity?