Is there an established method or protocol to load a specific protein inside extracellular vesicles or to integrate it in the vesicle membrane?
If you are talking about exosomes targeting then there are not so many examples of protein loading (most of the time people like to load siRNA or mRNA into exosomes). One example or protein loading is the following : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3077638/
There is a very good reason to target a protein of interest to the plasma membrane as it has a good chance to end up in exosomes or microvesicles, as the paper suggested by Nicolas Brouilly recommends. There is also an interesting case of the sequence motif EKPPHY at the C-terminus of insulin degrading enzyme (IDE), which presumably targets the protease to exosomes (or, as autors write, to the non-conventional mode of secretion). The paper also demonstrated that “mutation of this motif strongly affected the secretion of IDE.” I wonder whether someone tried to insert this motif to other proteins to see if this could promote targeting to exosomes. The paper on IDE:
http://www.jbc.org/content/286/26/22711.long
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