How to overcome the tension or frustration with others who are less organized or careless than you are?

Working with others usually good and build a massive amount of experiences, but sometimes there are some drawbacks of it. Careless, less organized, randomness, and commitment are some important...

30 October 2020 2,520 8 View

Tomato pollen internal membrane dye?

Hello everyone, I wonder if you can help to suggest any internal membrane dye that would enable me to stain the generative cell in tomato? unfortunately I have no marker tagged to the cell but I...

28 October 2020 2,335 3 View

Transient heat transfer through 2 walls and to air?

Dear All I am trying to simulate a problem that simplified as shown in the attached file. It has two different walls one inside the other and surrounded by air. I want to simulate the problem in...

14 October 2020 1,002 19 View

How many volume for media that we need to isolate exosomes from media?

Because i isolate 5 ml of culture media without serum and i got 1.2 ng miRNA . And i need 70 ng minimum that we can do PCR

28 September 2020 5,887 4 View

Is there any research points link Statistics and Big Data analytics that can be good points for research in PhD ?

Statistics and Big Data Analytics

02 August 2020 2,830 6 View

Which companies provide Molecular imprinted polymers ( MIPs) ?

19 July 2020 8,876 9 View

What are the differences between Renewable Energy and Energy Efficiency Technologies?

I found that energy efficiency deals with combustion efficiency, using biomass fuels and stuff like that. Can we link between wind energy and energy efficiency by some means?

07 June 2019 2,892 6 View

What are the best mesh quality criterion for inspection element quality?

I know about mesh density, skewness, wrap angles, and aspect ratio. Per literature, aspect ratio is a quick tool to define quality of mesh. Is there a rule of thumb for acceptable values of...

07 February 2019 7,145 4 View

Is Fmincon solver suitable for nonlinear ratio (I.e. NLP/NLP)?

I use fmincon to minimise weight to stiffness ratio. This ratio is Non linear. I tested this solver by using different initial points Xo. However, results are changeable and dependent on Xo. I am...

01 February 2019 3,069 3 View

What are the main considerations in injection mold designing and calculations?

I'd like to know the main parameters that should be considered in designing of injection mold. Also, I need to know a source for standardized calculations.

10 February 2018 6,412 3 View

Affordable source of Thyrotropin / bTSH?

I've been trying and failing to obtain cell-culture grade crude TSH (probably bovine) for propagation of laboratory thyroid cell lines (ie. FRTL5) - Merck / Sigma had a suitable product in their...

04 May 2019 6,718 1 View

Affordable source of Thyrotropin / bTSH?

I've been trying and failing to obtain cell-culture grade crude TSH (probably bovine) for propagation of laboratory thyroid cell lines (ie. FRTL5) - Merck / Sigma had a suitable product in their...

01 May 2019 1,335 1 View

Am working on a project "Comparative analysis between TSH and lipid profile of anemic patients". How do i calculate the statistical analysis?

Please i would need a guidance on how to cumulate results in statistical analysis and the best method i should apply.

02 March 2019 5,458 2 View

Siemens TSH3 ultra falsely underestimates TSH in South East Asians ?

Siemens diagnostics 3rd Gen TSH assay 'TSH3ultra' falsely underestimates TSH in case of a rare variant of TSH found mostly in South East Asians. Any experiences in this regard and any lab...

11 December 2018 6,357 0 View

How long can you store a reconstituted TSH sample in a refrigerator?

We have reconstituted human TSH hormone (Sigma), that we use in our immunoassay system. How long can we keep the same at 4 deg before any sort of loss in activity is observed? We do multiple test...

09 October 2018 4,450 0 View

What can be the reason for decreased ELISA reading of reconstituted TSH hormone?

We recently got TSH hormone from Sigma. On the first day, we ran our assay, and the results were fine. On the second day, the activity has somehow reduced by 90%. How is this possible? We...

07 August 2018 348 2 View

What would be the best coating buffer for covalent coating of TSH antibody (IEF Range: 5.8-6.9) on a functionalised PMMA surface?

We are optimizing the assay parameters for our TSH immunoassay. We had been using a 7.4 pH PBS buffer for antibody coating so far, but now want to evaluate if changing the pH of the coating buffer...

29 July 2018 1,144 2 View

What would be the best coating buffer for covalent coating of TSH antibody (IEF Range: 5.8-6.9) on a functionalised PMMA surface?

We are optimizing the assay parameters for our TSH immunoassay. We had been using a 7.4 pH PBS buffer for antibody coating so far, but now want to evaluate if changing the pH of the coating buffer...

06 July 2018 3,644 2 View

TSH ELISA kit?

I am looking for a human TSH ELISA kit, but the antibodies used in the kit could not from mouse. Are anyone know about that? please?

03 April 2018 1,518 4 View

Where to introduce this publication: P. Freychet et al, ?

I do not find this publication in research gate. Can I introduce the corresponding full text and in case how can I proceed. P. Freychet, J. Pelletier, G. Rosselin, Nicole Grenier, Christiane...

02 March 2018 644 2 View