Size of the Genomic DNA through Zymolyase method of yeast DNA preparation..
Unless you are immobilizing the cells in a matrix (such as agarose plugs) and digesting away all contaminants, all genomic DNA preps, regardless of the exact methodology, will actually consist of a population of sheared DNA molecules whose size range depends on the extent of shearing (due to pipetting, vortexing, or simply mixing). So, a) a genomic DNA prep will not have a single size, and b) it is impossible to predict in advance the size range of the prep.
Depending on the care you take, shearing of chromosomal DNA can be reduced but you will not get intact DNA corresponding to each chromosome. On a gel, you will always see smearing because of variability in DNA fragments.
If done carefully, bulk of the DNA will be in the range >40kb.
Thank you Alejandro Martin and Tausif Alam. But it seems you have not understood my problem correctly.
I don't bother if the chromosomes are broken because of shearing. Albeit there cant be exact size, because each chromosome gets broken randomly, still I just want to know the approximate size range of the broken chromosomes.
Because I want to ligate those long fragments with YCplac33 vector (after blunting the chromosome ends and cutting vector with SmaI).
Expecting your replies.
"Thank you Alejandro Martin and Tausif Alam. But it seems you have not understood my problem correctly."
Perhaps our mind-reading skills are not on par what you expected!
Given that the size of sheared DNA does not matter, pick the size you consider optimum for your work from a preparative gel, purify the DNA mixture and proceed with your experiment.
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