What is the simplest method to measure rat retinal protein kinase c? Are there any ELISA assays for the same?

More Ashlesha S Vaidya's questions See All

What is the meaning of *DO,ii,1,28,1 in ansys APDL command. Can anyone explain one by one parameters of comma marked?

It is a command of APDL for ansys transient problem.

03 May 2023 7,488 1 View

H2SO4 as a solvent for drugs in rats/mice?

Does anyone has experience in administering small molecules to rodents using extremely small volume of H2SO4 (like 0.02-0.05N). If possible please provide references for the same?

12 March 2023 5,482 0 View

Can pre-treatment with MitoSOX be given to cells for a time dependent estimation of mtROS?

We are looking to do a time dependent mtROS measurement after MPP+ treatment given to cells. Can anyone suggest if pretreatment with MitoSOX ( followed by washing) could be given before cells are...

22 August 2021 6,040 2 View

How can I compute the sound level from a pressure field?

My study topic is jet mixing noise. I am given a velocity field in Vx, Vy, Vz at x and y coordinates, and would like to find the pressure field, and from that, find the sound level (or at least...

15 March 2021 464 4 View

Interested in contributing to a Special Issue in Entropy (MDPI, IF 2.49)?

I am organizing a special issue on 'Entropy and Organization in Natural and Social Systems' in the journal Entropy published by MDPI (IF 2.49). This Special Issue is focused on understanding the...

30 November 2020 2,188 1 View

Where can I find a detailed database with info on how many people did COVID 19 patient meet before being symptomatic?

We are building a mathematical model for COIVD 19. We are looking for data with Patient ID, Age, Gender, Travel from and to, Date of arrival, a number of people the patient met after arrival,...

29 March 2020 8,173 4 View

Which fibroblasts are better for making a scaffold? Embryonic or Adult?

I want to use fibroblasts to synthesize ECM scaffold for wound care. Is it better to use embryonic fibroblasts or adult fibroblasts? And why?

10 February 2020 9,499 1 View

What is the prognosis of portal hypertension , Cirrhosis of Liver with Ascitis?

A case with portal hypertension and Cirrhosis since 4 years having Ascitis since 1 month. What will be the prognosis specific to this case and in general as well?

04 January 2020 1,124 17 View

Are there any general rank condition results on the controllability gramian?

I was looking for bounds on the controllability gramian matrix in integral form. Are there any known norms to compare different gramians?

26 April 2019 3,375 3 View

For how long the electrophysiological recordings (extracellular field recordings in the scaffer collateral) be done in the submerged bath chambers?

How long will the LTP last following an hour of baseline and induction via TBS in the submerged bath chambers for electrophysiology

20 April 2019 1,224 4 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

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Transfection in HEK293T cells?

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31 July 2024 9,892 4 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

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Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

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EDC carbodiimide and NHS easter 's half life among different pH?

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25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Pritesh Hargovindbhai Sabara

i don't know about any avalable ELISA kit for it, but you can cusomised it by giving order to any company?

Richard Presland

You need to know which type of PKC you are interested in (alpha, beta, gamma, delta, etc.). Merck/Millipore sell a number of different antibodies that crossreact with several mammals (see link below), but you would need to develop the ELISA yourself once you are satisfied with the quality of the antibody. Hope this helps.

http://www.merckmillipore.com/life-science-research/pathways-protein-kinase-c/c_FKub.s1OmfIAAAEj6OQU.HXl

Ashlesha S Vaidya

Thank you Richard. Its PKC beta I am interested in. This will surely help.