Does anyone has experience in administering small molecules to rodents using extremely small volume of H2SO4 (like 0.02-0.05N). If possible please provide references for the same?
How to calculate a,b, c coefficient using si/al and ca/(si+Al ) ratio? Example- ca/(si+al) ratio are 0.1, 0.2 and 0.3 also si/al ratio are 2.5,3.0,3.5,4.0,4.5 "a" coefficient are...
29 June 2023 9,778 1 View
How to calculate yield stress value in food products like a sauce?
29 June 2023 8,515 0 View
It is a command of APDL for ansys transient problem.
03 May 2023 7,488 1 View
We have taken return period of 2,5,10,25,50,100yr. and my question is to how we can select storm duration for design rainfall using Intensity-duration-frequency (IDF) curves.
28 September 2021 8,173 3 View
We are looking to do a time dependent mtROS measurement after MPP+ treatment given to cells. Can anyone suggest if pretreatment with MitoSOX ( followed by washing) could be given before cells are...
22 August 2021 6,040 2 View
My study topic is jet mixing noise. I am given a velocity field in Vx, Vy, Vz at x and y coordinates, and would like to find the pressure field, and from that, find the sound level (or at least...
15 March 2021 464 4 View
I am organizing a special issue on 'Entropy and Organization in Natural and Social Systems' in the journal Entropy published by MDPI (IF 2.49). This Special Issue is focused on understanding the...
30 November 2020 2,188 1 View
We are building a mathematical model for COIVD 19. We are looking for data with Patient ID, Age, Gender, Travel from and to, Date of arrival, a number of people the patient met after arrival,...
29 March 2020 8,173 4 View
I want to use fibroblasts to synthesize ECM scaffold for wound care. Is it better to use embryonic fibroblasts or adult fibroblasts? And why?
10 February 2020 9,499 1 View
A case with portal hypertension and Cirrhosis since 4 years having Ascitis since 1 month. What will be the prognosis specific to this case and in general as well?
04 January 2020 1,124 17 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View