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30 November 2020 1,941 1 View

What will be the effect on leakage inductance in the planar transformer for the LLC converter design?

I have analyzed on the effect on leakage inductance for the planar transformer, therefore came across the research paper and found the below formula for leakage inductance calculation: Also, i...

25 October 2020 8,871 3 View

Can we convert the graphene oxide films into rGO films without actually removing graphene oxide from the substrate?

What are the possible ways to convert GO to rGO?

19 October 2020 2,875 4 View

Suggest me a best filamentous m13 vector to generate scfv using phage display method?

i went through many literature to find out the exact viral vector but it was generally mentioned m13 vector in the papers not specific about the name, can anyone suggest me which m13 vector can...

18 October 2020 2,644 1 View

How to prepare MoS2 nanoparticles and 2D material using chemical method?

How to prepare MoS2 nanoparticles and 2D material using chemical method? Is MoS2 a transparent material, what about the absorbance of the MoS2?

12 October 2020 1,874 3 View

How to rank the random effect coefficients of an independent variable in presence of cross-level interactions in a Hierarchical Model?

I'm building a model that estimates the effect of a firm level variable (x) on its financial performance (y). It is a cross-classified three-level model with yearly measures of a firm in level-1...

03 October 2020 6,187 2 View

Can we detect neuroprotection, ischemic tolerance, ischemic pre conditioning using fmri ?

I want to know whether there are any non invasive techniques to detect neuroprotection in humans

02 October 2020 5,896 3 View

In substitutional solid solutions, do the substituting atoms form chemical bonds as they replace the atoms? How are they replacing the existing atom?

I do not understand how atoms replacing the base metal in substitutional alloys actually replace the base metal atoms and not form bonds.

20 September 2020 433 3 View

How to acheive collimate light rays using the LED Spot light?

I am using the light source as LED spotlight 50W (MFG part no: MASTER LED ExpertColor LED MR16 ExpertColor 7.2-50W 927 10D ). Hence i am planning for lens mechanism to convert the divergence...

24 July 2020 9,527 2 View

Where can I find a detailed database with info on how many people did COVID 19 patient meet before being symptomatic?

We are building a mathematical model for COIVD 19. We are looking for data with Patient ID, Age, Gender, Travel from and to, Date of arrival, a number of people the patient met after arrival,...

29 March 2020 7,954 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

12 January 2021 6,260 1 View

Does someone had trouble with fixable life/dead in endometrial flow cytometry?

Hi. I am doing flow cytometry of endometrial cells (read epithelial and fibroblast). The antibodies I need to use are both intracellular. In the same time I want to get only life cells. Thus I...

07 January 2021 6,473 3 View

Why can I not detect other targets than GAPDH?

Hello, we're trying to detect Keratin 14 and 15 in Keratinocytes as well as FN-1 and CD90/THY1 in Fibroblasts from human skin. As reference gene GAPDH and Vinculin are used. In our first...

07 January 2021 6,652 3 View

How can I take an electrical conductivity test from a nanofibrous scaffold?

I have a nanofiber scaffold for tissue engineering. When I did the electrical conductivity test, the ohmmeter reports a wide range of values (from 300 kOhm to 20 MOhm). I placed the scaffold...

06 January 2021 2,232 1 View

What are these called?

Upon tissue plating for cell isolation, I start to see fibroblasts and after a while these 3D structures start to form. Are they called stroma? Someone in my building told me that she calls them...

30 November 2020 8,685 4 View

Is there any technique to reduce carbonate precentage in bone sample (bovine hydroxyapatite)?

Dear colleagues, I am a researcher working with bovine hydroxyapatite. I want to reduce the percentage of carbonate on the sample. Is there any suggested techniques for this? I am measured the...

19 November 2020 3,828 3 View