Which fibroblasts are better for making a scaffold? Embryonic or Adult?

11 February 2020 1 9K Report

I want to use fibroblasts to synthesize ECM scaffold for wound care. Is it better to use embryonic fibroblasts or adult fibroblasts? And why?

Ron Hrstka

What species?

In general fetal (not embryonic) fibroblasts are preferable to adult fibroblasts as they should be able to tolerate more population doublings in culture. Murine pups at 12.5 to 13.5 dpc are ideal for fibroblast preps.

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