why do we need whole genome sequencing of organisms?
Thanks Umair for your valuable response
I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...
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Article: Integrating intravenous frusemide treatment into the community for heart failure patients Dear Sir/Madam I am one of the authors of this article but the citation went to a wrong person....
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ANSYS Mechanical APDL is one of the numerical simulation software.
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I would like to present the following testimony, which was provided by a foreign doctorate student attending Bahir Dar University: "Dear Professor Solomon, Greetings. I am writing to extend my...
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Do some journals accept the manuscripts of scholars, who have a lot of publications, more quickly than beginners, who have no or less number of publications, irrespective of the quality of the...
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There appears to be arguments for and against this position.
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I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
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I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
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I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
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Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
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Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
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Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
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Gene sequencing related trouble shooting
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Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
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In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
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