I am looking at Puccinia lagenophorae and its affects on 3 different species of Groundsel for my dissertation. I want to conduct a PCR on the fungus for identification purposes.However I cannot find any methodology on the isolation of the fungi so that I do not culture any Type 2 fungi, as these would render my project unsafe for undergraduate level.
Obligatory fungus such Puccinia are difficulties that can be cultivated in Petri dishes. I have an idea that can be debatable: you take the leaves of the host plants of the pathogen puccinia, you grind and you filter (take the juice) you add the agar (20g / l) and can be sucte (glucose or dextrose ...)
Hello Megan Burt
Rust fungi like Puccinia are obligate parasite so it is need either living tissues ( host plant) or axenically in liquid culture, could be more pronounced on media containing pollen grains only and/or inositol. I attached references possibly useful for answering your question. Good luck
Hi Megan,
What is your goal for culturing this rust fungus? Unless you have a different objective, you can directly crushing the urediospores and conduct conventional PCR method on genomic DNA. As Dr. Al-Janabi stated earlier, rust fungi are obligate parasites and need living tissue for their infection process many rust fungi have been cultured. To-date, all rust fungi which were axenically cultured on defined medium (MS medium used for plant tissue culture) are considered ecologically obligate parasites.
I suggest following the following method of the very same rust fungus
that you are working with by following the following reference :
Taxonomy and phylogeny of Puccinia lagenophorae: a study using rDNA sequence data, morphological and host range features Markus Scholler & Matthias Lutz & Alan R. Wood & Gregor Hagedorn & Mechthilde Mennicken . Mycol Progress (2011) 10:175–187 DOI 10.1007/s11557-010-0687-0
Good luck with your research work
Simon
Dear Dr. Shamoun
I agree with your suggestions, in fact, I am astonishing about her interesting for isolation of this fungus, it is quite enough for PCR process to collect the rust inoculum directly from infected plants, but maybe she likes to do a particular study on rust culture. With my best regards
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