I want to isolate Intestinal epithelial cells and intestinal myofibroblasts. In some protocols, I found they use DMEM containing sorbitol to wash cells after collagenase/dispase incubation. Do they use sorbitol to neutralize collagenase/dispase?
But in these two websites, none of them mentioned that collagenase/dispase can be inhibited by sorbitol.
http://www.stemcell.com/zh-CN/Products/All-Products/Dispase-1-mgmL.aspx
http://www.worthington-biochem.com/cls/default.html
Thanks a lot.