Ruizhi Tang

4 Questions 3 Answers 0 Followers

Questions related from Ruizhi Tang

What is the role of sorbitol in the collagenase and dispase treatment?

I want to isolate Intestinal epithelial cells and intestinal myofibroblasts. In some protocols, I found they use DMEM containing sorbitol to wash cells after collagenase/dispase incubation. Do...

03 December 2014 4,054 1 View

Serum starvation for Mouse embryonic fibroblasts (MEFs)?

I treat MEFs without serum for 24 hours, but I found many light dots in the cell culture. Are they dead cells or apoptotic bodies? Is 24 hours too long? I found some groups even treating cells...

17 October 2013 6,661 2 View

How to introduce new restriction recognition sites in vector?

Hello,everyone.I want to introduce EcoR1 and Not1 sites into the original Xba1 site in PGL3 vector.How can I do that?Are there some references about that? Thanks.

17 December 2010 5,273 3 View

How to introduce new restriction recognition sites in vector?

Hello,everyone.I want to introduce EcoR1 and Not1 sites into the original Xba1 site in PGL3 vector.How can I do that?Are there some references about that? Thanks.

17 December 2010 7,734 1 View