Can anybody explain the role of hydrocortisone for the growth of MCF 10A cells? Is it necessary for the culture of the cells in DMEM/F12 HAM medium? Can I culture the cells without hydrocortisone?
Hi I have AGS cell lines in culture for our experiments. Recently, I started facing fungal/ yeast contamination of the AGS cell line in culture continuously, once they are 90% confluent. The...
11 December 2018 1,542 11 View
I want to synchronize the normal and cancer liver cells in G1 phase. Can lovastatin and serum deprivation methods be used for synchronization of the hepatocytes? Can anybody help me with the...
01 February 2017 1,506 5 View
I have to use pre-coated flasks for some of my cell lines (BEAS-2B, THLE-2). Can anybody help me with the flask coating (BSA, collagen and fibronectin)? For how long can I store the prepared...
04 May 2016 4,211 3 View
I'm preparing the samples for my experiment for TEM. I did the resin embedding step. After adding the pure resin I centrifuged the cells at 1,500 rpm 5 minutes but could't pellet it well. The...
01 February 2016 1,008 4 View
I want to leave the samples for electron microscopy in gluteraldehyde fixative over the weekend and continue the process next week. Will it affect the samples? How long can the samples be left in...
01 February 2016 9,892 6 View
I want to know how to determine the concentration / amount of nanoparticles that should be used for the experiment
31 December 2015 8,073 11 View
I want a procedure for the extraction and estimation of total lipids from cultured animal cells. I'm mostly interested in protocols using spectrophotometric assays.
11 December 2015 3,652 3 View
I want to know if there is a PCR test that helps to identify the 293T cells.
10 November 2015 1,981 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View