Shaima Sirajudeen

9 Questions 13 Answers 0 Followers

Questions related from Shaima Sirajudeen

Have anybody faced repeated contamination of AGS cell line?

Hi I have AGS cell lines in culture for our experiments. Recently, I started facing fungal/ yeast contamination of the AGS cell line in culture continuously, once they are 90% confluent. The...

12 December 2018 1,491 11 View

How can I synchronize the liver cells?

I want to synchronize the normal and cancer liver cells in G1 phase. Can lovastatin and serum deprivation methods be used for synchronization of the hepatocytes? Can anybody help me with the...

02 February 2017 1,463 5 View

What are the steps for flask coating?

I have to use pre-coated flasks for some of my cell lines (BEAS-2B, THLE-2). Can anybody help me with the flask coating (BSA, collagen and fibronectin)? For how long can I store the prepared...

05 May 2016 4,161 3 View

What is the role of hydrocortisone in the culture of MCF 10A cells?

Can anybody explain the role of hydrocortisone for the growth of MCF 10A cells? Is it necessary for the culture of the cells in DMEM/F12 HAM medium? Can I culture the cells without hydrocortisone?

05 May 2016 8,039 0 View

Is it a good practice to leave the samples in gluteraldehyde fixative over a few days and continue processing for electron microscopy?

I want to leave the samples for electron microscopy in gluteraldehyde fixative over the weekend and continue the process next week. Will it affect the samples? How long can the samples be left in...

02 February 2016 9,841 6 View

I embedded the samples for TEM in a day old resin and can't pellet it. Is there a way to save my samples?

I'm preparing the samples for my experiment for TEM. I did the resin embedding step. After adding the pure resin I centrifuged the cells at 1,500 rpm 5 minutes but could't pellet it well. The...

02 February 2016 966 4 View

How to determine the concentration of nanoparticles to be used in experiments?

I want to know how to determine the concentration / amount of nanoparticles that should be used for the experiment

01 January 2016 8,043 11 View

What is the procedure for extraction and estimation of total lipids from cultured animal cells?

I want a procedure for the extraction and estimation of total lipids from cultured animal cells. I'm mostly interested in protocols using spectrophotometric assays.

12 December 2015 3,613 3 View

Is there a PCR specific for 293T cells?

I want to know if there is a PCR test that helps to identify the 293T cells.

11 November 2015 1,946 2 View