Hello,
I am trying to isolate endothelial progenitor cells from umbilical cord blood and currently follow the protocol published under the title "An in vivo experimental model for postnatal vasculogenesis. (http://www.sciencedirect.com/science/article/pii/S0076687908030139)". Here they use EGM-2 medium without hydrocortisone.
I found a recent patent (http://patents.justia.com/patent/20050266556) in which they carry out a similar isolation but they used complete EGM-2 medium which contains hydrocortisone.
What role does hydrocortisone have in an endothelial progenitor cell isolation or in more generic form, what role does it play in a egm-2 medium?
Thank you for looking into this and would love to hear from you all.
Hi Prashanth,
Hydrocortisone is usually added to endothelial media, not only to EGM-2 (which is proprietary) but also to, for instance MCDB 131. It tends to prevent stressing of endothelial cells a little and sensitizes cells to growth factors, especially EGF.
If you like to brush up on ancient endothelial cell culture media history, have a look at the attached paper. It's by Richard Ham (famous for the F12 supplement mix) and describes the making of MCDB 131. He shows that hydrocortisone sensitizes cells to EGF and increases proliferation.
Also have look at the publication date... Funny how some knowledge gets lost over the generations.
Dear Prashanth,
Hydrocortisone, although not pro-angiogenic itself, has a beneficial effect on angiogenesis (Goding, 2009). Hydrocortisone increases the number of branches in the vascular-like network. However, it produced cell aggregates at high concentrations whereas a lack of hydrocortisone induces a sparse vascular-like network. Hydrocortisone acts as a mitogen in the vascular model.
Goding, A. M. (2009). The effect of hydrocortisone on angiogenesis.
BIOS 80, 59-65. http://dx.doi.org/10.1893/011.080.0202
For more information on hydrocortison use in EGM-2 medium, please see the publication contained in the following link:
http://altweb.jhsph.edu/altex/32_2/Huttala.pdf
Hoping this will be helpful,
Rafik
Thank you so much for your help and links Dr. Rafik! This would help a lot.
Hi Prashanth,
Hydrocortisone is usually added to endothelial media, not only to EGM-2 (which is proprietary) but also to, for instance MCDB 131. It tends to prevent stressing of endothelial cells a little and sensitizes cells to growth factors, especially EGF.
If you like to brush up on ancient endothelial cell culture media history, have a look at the attached paper. It's by Richard Ham (famous for the F12 supplement mix) and describes the making of MCDB 131. He shows that hydrocortisone sensitizes cells to EGF and increases proliferation.
Also have look at the publication date... Funny how some knowledge gets lost over the generations.
Appreciate your help Dr. Hendrik. It definitely is surprising that some of the basic research is sometimes the hardest to find. Thanks again for the paper as well.
Hi all,
I am a Research Associate at Umass medical school, working with similar culture system. I wanted to know concentration of hydrocortisone recommended in culture medium, if I decide to use it/add it separately?
I would really appreciate any suggestions, Thank you!!
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