8 Questions 14 Answers 0 Followers
Questions related from Anand Vivek Desai
I performed a fluorometric experiment with two different cell lines. I had four conditions per experiment, and four "sub-conditions" per condition, wherein I add three different chemicals (the...
09 November 2020 5,275 3 View
Hi all, I am planning to do a RT-PCR followed by qPCR starting from 100 ng of total RNA. The way we do it in our lab is we dilute the 20 uL of RT reaction containing 1 ug of total RNA 1:4 and use...
25 July 2017 8,059 5 View
Hi all, I did this PCR with my reference and test DNA. I had tested the two primer pairs with my reference DNA and they worked pretty well. I ran the PCR and obtained bands with different band...
25 January 2017 9,880 3 View
Hi all, I have been optimizing Surveyor nuclease assay to detect indels in the genomic DNA of Cas9 transfected HEK cells. To begin with, I am using 300 ng and 400 ng of total DNA (Control DNA...
23 January 2017 3,011 9 View
Hi all, This seems to be a fundamental thing but I have this doubt. I have biological replicates of a timecourse experiment with a particular treatment. I wanted to know if I can combine both to...
17 January 2017 4,838 3 View
I have a RNA sample with 300 ng/uL of concentration and 260/280 as well as 260/230 ratios higher than 1.9. I ran an agarose gel to test the quality of my RNA sample and my loading buffer had a...
12 January 2017 8,912 14 View
I have three tubes of 5 mL each of starter culture of overnight grown bacteria with three different plasmids stored at 4 degrees. I was curious if these cultures can be used to prepare glycerol...
03 October 2016 4,172 3 View
I have three plasmids with identical Cas9. EGFP is a selectable marker in all the plasmids. The plasmids are identical in all the aspects except for one; each one has a different guide RNA cloned...
07 September 2016 5,610 3 View
