What is the reason that we can not get stable peak for both low and high PH for our sample in Lc-Ms-Ms?

More Aysana Sadra's questions See All

What are the differences between systematic scoping review and scoping review?

Hello every one, I want to understand the differences between a systematic scoping review and a scoping review based on the all aspects of a review study. Thank you in advance.

11 June 2023 8,526 0 View

Can any one help me with a protocol to freeze-dry cell laden hydrogel scaffolds for SEM analyses?

Articles have only mentioned that cell-laden hydrogel scaffolds were lyophilized before SEM analyses for cell adhesion. However, no details were mentioned.

05 June 2022 3,182 2 View

Does the surface tension of non-Newtonian Liquids change like their viscosities?

We know that the viscosity of non-Newtonian liquids changes when shear is applied on them. On the other hand, some correlations are available about the relation between viscosity and surface...

30 November 2021 4,325 4 View

Hello everyone. I have a list of mRNAs and I would like to identify transcription factors regulating them. Is there any way to do that?

Specially, I am looking for a database or software which give me the name of transcription factors and mRNAs which are being regulated by them.

28 October 2021 5,257 3 View

I am working on lncRNAs and mRNAs and I have a list of them. Is there any database or other ways to identify their host genes?

I am looking for host genes coding these lncRNAs and mRNAs.

17 July 2021 6,352 2 View

Is ANSYS Fluent good for simulation of a three phase reactor(stusy of its hydrodynamics or mass transfer...)?

Hello everybody, I would like to simulate a three phase reactor and study its hydrodynamics. I would like to know which type of software is better? I have a good command of ANSYS Fluent and I am...

10 February 2021 4,574 3 View

What is the maximum walking acceleration/deceleration over a very short time period (e.g., 0.02, 0.1, 0.5 sec)?

I'm trying to simulate walking speed and I need to have an idea that how walking speed is changing over very short period of time (e.g., 0.02,0.1,0.2, and 0.5 sec). I know that average normal...

29 August 2016 760 4 View

What is the maximum instantaneous walking acceleration rate?

Is there any reference on maximum walking acceleration rate for a very short period of time? (e.g., 0.02 sec, 0.1 sec)?

25 August 2016 5,258 2 View

What is the maximum instantaneous walking speed for normal individuals?

We know that maximum walking speed is around 1.5 m/s for normal individuals. This speed is obtained based on tracking individuals in a time duration of 1 sec. Is there any reference to show that...

25 August 2016 306 4 View

What is the best filter size we can use in our solvents for LC-MS/MS ?

LC-MS/MS system consisted of the 460-LC auto samplers, 212-HPLC Chromatograph Pumps. Solvents are water and methanol + 0.1 % formic acid.

15 October 2013 4,916 1 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Hello dear researchers. I would like to know what is a normalized mass in an EDX analysis?

EDX analisys

31 July 2024 5,947 2 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

Vijaayan Krishnan

The positive or negative peak stability depends on the chemistry of the molecule and the nature of the solvent system ie the buffer

Aysana Sadra

Dear Varun,

What is the relation between temperature and melting point? what do you mean by checking melting point?

what is the best buffer for L-ascorbic acid ? I try to stabilize it in low PH (PH

Temp n extraction cone volt and can u provide me the str.

sorry,I did not understand this sentence.

I wanna know capillary voltage is cone voltage?