I spin coated polystyrene solution (5 mg/ml) but there's defect like bubble.
I don't know the reason.
Please help me.
I ran PCR using COI universal primers on DNA extracted from lice. I added 25ul of 2x master mix, 5ul template, and 1ul each of 20uM F and R primers, with the remaining volume made up with DW to a...
20 April 2024 1,565 4 View
Can anyone advise me on how to separate micas(mostly muscovite) from carbonaceous black slates comprising quartz, mica, and coals?
04 February 2024 8,721 1 View
Hello all, I'm currently immersed in research involving the Rosa26-tdtomato (Ai9) mouse line. We performed scRNA seq and analysis focusing on tdtomato-expressing cells but faced a challenge with...
01 February 2024 6,390 0 View
In the field of geology, How can we delineate the distinctions between 'geochemical processes' and 'geochemical mechanisms'?
10 September 2023 6,301 5 View
Could you please explain the relationship between a rift basin, a rift valley, and the intrusion of seawater in the geological process?
05 September 2023 5,895 3 View
Hi researchers, I have a snRNA Seq dataset, and I am analysing it using R Seurat package. My task is to find out if the expression of a certain genes in the cells leads to differences in the...
24 August 2023 4,831 0 View
How to distinguish between a bed and a layer through outcrop survey and thin section observation?
20 July 2023 8,030 6 View
If an iron former with a different lead isotope composition is injected, will there be Pb and Sr isotope fractionation during the high-temperature smelting process?
18 July 2023 800 0 View
Hi, I'm a student under doctor course. These days, I have unsolved problems... That are captures about picro-sirius red staining on mouse renal frozen section. There are some red signals in...
11 July 2023 4,639 3 View
Hello :) I would like to perform co-sputtering with elemental chips like the attached image. And I wonder how can I attach the chips on the target surface? * Is it okay if I just put it on? Or...
31 May 2023 7,350 16 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
I am currently using CASTEP version 2020. When I want to run optical properties calculations with spin-orbit coupling(SOC), I encounter the following error in a pop-up window before the job gets...
02 August 2024 2,890 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,294 3 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
In the context of Electron Spin Resonance (ESR) spectroscopy, under what conditions is an absorption spectrum considered to be a single absorption spectrum?
25 July 2024 2,325 1 View