I spin coated polystyrene solution (5 mg/ml) but there's defect like bubble.
I don't know the reason.
Please help me.
I want to label my target miRNA and see the miRNA trafficking. But I don't want to use any plasmid or radioactive label. I need the fluorescent label that will not affect the length of my...
06 December 2020 8,471 3 View
I accidentally resuspended my stock primers (100 uM) in tris-Cl (pH 8.0) 1 M instead of 10 mM. I do PCR with 20 uM dilution from the stock. Will the high concentration of Tris-Cl inhibit PCR? I...
12 July 2020 2,971 10 View
Hot KOH destroy many materials like alumina or glass.. So, can I use zirconia(ZrO2) boat for KOH activation of carbon nanoparticle experiments?
09 April 2020 800 1 View
Hi, I am working on the home advantage. And examining the difference of a player's performance between playing at home and away. However, the sample size is different because the player played...
24 February 2020 7,053 3 View
I have some 3 year old PPFE slides of human brain that have been reacted with primary antibodies then reacted with Alexa Fluor secondary antibodies. The DAPI on the slides is still visible but the...
02 May 2019 7,390 2 View
Hi I am carrying out a study investigating the association between age and password strategies among online users. I have collected my data, and inputted it on SPSS, however, I am not sure which...
10 December 2018 1,002 7 View
Let's say I have an array of receptors. Given a peptide, can protein-peptide docking be used in predicting the peptide's target among the receptors? If so, how? If not, why? Can you recommend me...
24 July 2018 3,569 4 View
Good day all! I would greatly appreciate help with the following issue: Our lab is trying to characterize mouse lymphocytes for Tbet, EOMES, and PD-1. When I ran the samples the first time, the...
28 March 2018 1,225 3 View
If the mounting is delayed for a day or two, would the specific binding being greatly reduced?
05 December 2017 4,103 3 View
15 October 2017 9,870 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...
01 March 2021 8,220 2 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....
28 February 2021 7,967 3 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax
28 February 2021 6,685 2 View
I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?
28 February 2021 274 2 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...
27 February 2021 1,829 3 View