Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

Regarding to presentation of pairwise comparison of data?

22 January 2021 8,648 1 View

What is the main advantage of using observer based fault tolerant controller(FTC) for sensor fault tolerance?

I am working on FTC for handling sensor faults in the system. I have read about observer-based FTC schemes which use error threshold-based logic between the states and estimated states. If a...

10 January 2021 5,771 2 View

Can a luenberger observer (even a modified version) be effective for a nonlinear affine system?

I want to know whether a luenberger observer or a modified form of it be effective for estimating the states of a system of the following forms: 1. x_dot = f(x,u,d); y = x 2. x_dot = f(x,u); y =...

20 December 2020 1,392 4 View

How much Tensile strength and hardness can be acheived in Aluminium 7050 MMC with B4C reinforcement?

I have conducted research on characterisation of Al7050 + B4C by electromagnetic stir casting. The alloy employed in the process is casted alloy and the Composite too is casted. How much strength...

16 November 2020 9,226 3 View

Why whitefly prefer not to infest Pigeonpea?

Whitefly, Bemisia tabaci is pest of broad host range. In pulse crops, it generally infests greengram, blackgram and cowpea but not Pigeonpea. There might be some biochemical or morphological...

09 November 2020 9,199 3 View

Can u suggest how to identify the matrix material and reinforcement particle distribution in the MMC of scanning electron miroscopy (SEM) images?

How to identify the reinforcement particle behavior in SEM images

09 November 2020 8,664 3 View

Can someone help me in designing off-MPPT mode in a Standalone DC Microgrid?

I am working on voltage control of a Standalone DC Microgrid consisting of Single PV source, a battery and loads. The PV is interfaced through Buck converter to the DC bus, the battery is...

01 October 2020 7,071 2 View

Modeling pure shear loads in Abaqus?

I am trying to simulate a pure shear loading on a cubical block with a single brick element. While I am simulating the other stress components are not getting zeros. Can anyone suggest to me how...

22 September 2020 7,649 3 View

Can anyone suggest any introductory book on tensor analysis and tensor calculus for continuum mechanics?

I have searched google. But it would be more helpful to get suggestions from personal experience.

14 September 2020 5,426 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View