What is the reason for bad results (No amplification curve) in miRNA amplification?

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Maurice Ekpenyong

Hello, I gather that mncl2 in poly A tailing kit interfers with the cDNA synthesis process. If you clean up the sample after poly A tailing, I believe your results will be better. You could also adopt another method like Signature Sequence Amplification Method (SSAM). The document is attached for protocol details.

Sri Vithya

@Maurice.. Thanks for your reply. But I am using stem-loop primer for my work. I am using Thermo Fischer cDNA synthesis kit for RT process. I wanna know am I doing it wrong. May I know the Do's and Don'ts while performing miRNA amplification ?