Dear Sri,

(1) D + 2M = M2D; where D and M are the drug and metal, respectively.

(2) K =[M2D] / [D] x [M]2

(3) [M2D] = K x  [D] x [M]2

You can determine [D] and [M] by a spectrophotometric method, NMR , HPLC or etc

Substitution on the values of K, [D] and [M] in equation 3 gives you the concentration of the complex, [M2D].

For more on this topic, please use the following link:

https://encrypted.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0ahUKEwjDp9y10a7NAhXKvRoKHYgZDmUQIAgxMAI&url=http%3A%2F%2Fwebcache.googleusercontent.com%2Fsearch%3Fq%3Dcache%3ARjq7qkOMsX0J%3Achemwiki.ucdavis.edu%2FCore%2FInorganic_Chemistry%2FCoordination_Chemistry%2FComplex_Ion_Chemistry%2FThe_Formation_of_Complex_Ions%2B%26cd%3D3%26hl%3Den%26ct%3Dclnk%26gl%3Dus&usg=AFQjCNFJ0eL5r9xLclPZZj_yBVN0n9te6A&sig2=GyqT8uw7u9x74xIDyBBy4A

Hoping this will be helpful,

Rafik

 Thank u very much sir!! Will go through it and let you know sir.