Dear All,
What is the ratio of FBS to trypsin to inactivate/neurtrilize trypsinization in cell culture?
In some protocol, the ratio of 10%FBS media to 0.25%trypsin-EDTA is 1:1 or 2:1. ATCC guide uses 3:1. So what is minimum or the best ratio of FBS to inactivate trypsin.
Thank you very much
Cheerio,