03 September 2015 4 4K Report

Dear All,

What is the ratio of FBS to trypsin to inactivate/neurtrilize trypsinization in cell culture?

In some protocol, the ratio of 10%FBS media to 0.25%trypsin-EDTA is 1:1 or 2:1. ATCC guide uses 3:1. So what is minimum or the best ratio of FBS to inactivate trypsin.

Thank you very much

Cheerio,

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